Thermo Fisher Scientific Phospho-MECP2 (Ser80) Polyclonal Antibody
MECP2 단백질의 Ser80 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin) 검증 완료. 인간, 생쥐, 랫트 반응성. PBS/glycerol buffer에 보관하며 -20°C에서 안정. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MECP2 (Accession P51608), corresponding to amino acid residues around phosphorylated Ser80. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816154 |
Product Specific Information
This antibody detects endogenous levels of MeCP2 only when phosphorylated at Ser80.
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a major modification in eukaryotic genomes essential for mammalian development. It can repress transcription from methylated gene promoters. Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation. It is dispensable in stem cells but essential for embryonic development. Mutations in the MECP2 gene cause most cases of Rett syndrome, a progressive neurological developmental disorder and a common cause of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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