Thermo Fisher Scientific Phospho-Vimentin (Ser83) Polyclonal Antibody
인간, 생쥐, 랫트에서 인산화된 Vimentin Ser83을 특이적으로 검출하는 폴리클로날 항체. Western blot과 IHC(파라핀) 실험에 적합. 고순도 정제 및 PBS/glycerol 저장 버퍼로 안정적이며 연구용으로만 사용 가능.
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Thermo Fisher Scientific Phospho-Vimentin (Ser83) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human VIM (Accession P08670), corresponding to amino acid residues around phosphorylated Ser83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816152 |
Product Specific Information
Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser83.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by a protein kinase leads to breakdown of intermediate filaments and activation of ATP- and myosin light chain-dependent contractile events. These cytoskeletal changes facilitate lipid droplet interaction with mitochondria, enhancing cholesterol transport and steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-104677_Vimentin_P08670-1_Rabbit.svg, PA5-104677_Vimentin_P08670-1_Rabbit_PDP.jpeg)
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