Thermo Fisher Scientific Phospho-Calnexin (Ser564) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Calnexin only when phosphorylated at Ser564.

Target Information

Calnexin, also referred to as IP90, p88, and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins.
Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes.
It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble ER resident proteins such as GRP78, GRP94, and protein disulfide isomerase.
Integral membrane ER resident proteins like calnexin often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.