Thermo Fisher Scientific Phospho-KLF4 (Ser254) Polyclonal Antibody
KLF4 단백질의 Ser254 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 인간, 생쥐, 랫트 반응성. 고순도 정제 및 안정한 PBS/glycerol 저장 용액 형태.
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Thermo Fisher Scientific Phospho-KLF4 (Ser254) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human KLF4 (Accession O43474), corresponding to amino acid residues around phosphorylated Ser254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816155 |
Product Specific Information
Antibody detects endogenous levels of KLF4 only when phosphorylated at Ser254.
Target Information
Kruppel-like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), it belongs to a family of conserved DNA-binding zinc finger transcription factors.
KLF4 plays key roles in cellular pathways such as endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle control. As one of the four Yamanaka factors, it is crucial for reprogramming differentiated cells into pluripotent stem cells.
Nuclear localization correlates with oncogenic activity, whereas cytosolic localization is associated with tumor suppression.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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