Thermo Fisher Scientific CD276 (B7-H3) Monoclonal Antibody (2H5)
CD276(B7-H3) 항원을 인식하는 Mouse Monoclonal Antibody(2H5)로, Human 시료에 반응합니다. WB, IHC, ICC, Flow Cytometry, ELISA 등 다양한 응용에 적합합니다. Protein A 정제, PBS+50% glycerol 보존, 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H5 |
| Immunogen | Extracellular domain of human B7-H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2890292 |
Product Specific Information
Positive controls that may be used include:
- HEK293 Cell Lysate
- HEK293 Cell Slide
Target Information
CD276, also known as B7-H3, is a member of the B7 family of co-stimulatory molecules. It is a type I transmembrane protein that induces proliferation of CD4⁺ and CD8⁺ T cells, enhances cytotoxic T cell generation, and selectively stimulates interferon gamma production. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines and is also widely expressed in peripheral tissues such as heart, kidney, testis, and colon. In humans, CD276 exists as two isoforms due to gene duplication and differential splicing. CD276 has potential therapeutic relevance in regulating immune responses to cancer, particularly in combination with anti-angiogenic therapies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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