Thermo Fisher Scientific HAT Tag Monoclonal Antibody (4A6F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

4A6F3

Immunogen

HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2890553

Target Information

HAT Tag (Histidine Affinity Tag) is a peptide epitope (KDHLIHNVHKEFHAHAHNK) used in protein expression and purification. In comparison to the His tag, it is rich in histidines and has high affinity for immobilized metal ions such as cobalt, nickel, and zinc. However, the sequence is naturally-occurring, featuring non-adjacent His residues that result in a lower overall charge than tags with consecutive histidines. HAT tag antibodies are available for the detection of tagged fusion proteins is meant to resemble the solubility of wild-type proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.