Thermo Fisher Scientific CD276 (B7-H3) Monoclonal Antibody (4H3)
CD276(B7-H3) 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 인간 시료 반응성이며, 단백질 A로 정제된 액상 형태입니다. 면역세포 활성 연구 및 종양 면역반응 분석에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H3 |
| Immunogen | Extracellular domain of human B7-H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2890293 |
Product Specific Information
Positive controls that may be used include:
- HEK293 Cell Lysate
- HEK293 Cell Slide
Target Information
CD276, also known as B7-H3, is a member of the B7 family of co-stimulatory molecules. It is a type I transmembrane protein that induces proliferation of CD4+ and CD8+ T cells, enhances cytotoxic T cell generation, and selectively stimulates interferon gamma production.
Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines and is widely expressed in peripheral tissues including heart, kidney, testis, and colon.
In humans, CD276 exists as two isoforms resulting from gene duplication and differential splicing. It has therapeutic potential for regulating cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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