Thermo Fisher Scientific NOX2 Monoclonal Antibody (NL7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

NL7

Immunogen

Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in octyl glucoside) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NOX2,uniProtId:P04839-1,ncbiNodeId:9606,antigenRange:1-570,antigenLength:570,antigenImageFileName:MA5-18051_NOX2_P04839-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18051_NOX2_P04839-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539434

Product Specific Information

This antibody is specific for NOX2/gp91phox (aa 498-507, EKDVITGRKQ).

Target Information

Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.