Thermo Fisher Scientific NOX2 Monoclonal Antibody (54.1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is specific for NOX2/gp91phox (aa 382–389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).

Western blot results show a band at 65 kDa, which matches the predicted molecular weight.
For IHC applications, perform heat-mediated antigen retrieval with citrate buffer (pH 6) prior to staining.

Target Information

Mox1 and the glycoprotein gp91-phox are essential components of the NADPH oxidase complex, which generates superoxide in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells.
The complex includes membrane-bound flavocytochrome subunits gp91-phox and p22-phox, and cytosolic proteins p47-phox and p67-phox.
These subunits also act as surface O₂ sensors initiating cellular signaling under hypoxic conditions.
Mox1 and gp91 share identical C-terminal sequences but differ in expression patterns: gp91-phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, while Mox1 is mainly found in the colon, with low levels in the uterus and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.