Thermo Fisher Scientific NOX2 Monoclonal Antibody (54.1)
NOX2/gp91phox 단백질을 검출하기 위한 Mouse monoclonal 항체. Western blot, IHC, ICC/IF에 적합. Human 및 Rat 시료에서 반응. Protein G 정제 및 PBS buffer에 보관. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 54.1 |
| Immunogen | Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539435 |
Product Specific Information
This antibody is specific for NOX2/gp91phox (aa 382–389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).
Western blot results show a band at 65 kDa, which matches the predicted molecular weight.
For IHC applications, perform heat-mediated antigen retrieval with citrate buffer (pH 6) prior to staining.
Target Information
Mox1 and the glycoprotein gp91-phox are essential components of the NADPH oxidase complex, which generates superoxide in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells.
The complex includes membrane-bound flavocytochrome subunits gp91-phox and p22-phox, and cytosolic proteins p47-phox and p67-phox.
These subunits also act as surface O₂ sensors initiating cellular signaling under hypoxic conditions.
Mox1 and gp91 share identical C-terminal sequences but differ in expression patterns: gp91-phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, while Mox1 is mainly found in the colon, with low levels in the uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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