Thermo Fisher Scientific IL-6 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Thermo Fisher Scientific IL-6 Polyclonal Antibody는 마우스 IL-6 검출용 항체로, Western blot 및 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도의 재조합 마우스 IL-6로 면역하여 제작되었습니다.
- 카탈로그번호
- 500-P56BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-6 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions |
| ELISA | 0.25–1.0 µg/mL | Detects 0.2–0.4 ng/well of Recombinant Murine IL-6 when used with PeproTech Polyclonal Anti-Murine IL-6 (500-P56) |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Murine IL-6 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930633 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
For detection of Murine IL-6, use 0.25–1.0 µg/mL of this antibody (100 µL/well). When paired with PeproTech Polyclonal Anti-Murine IL-6 (500-P56) as the capture antibody, detects at least 0.2–0.4 ng/well of Recombinant Murine IL-6.
Western Blot:
For detection of Murine IL-6, use 0.1–0.2 µg/mL of this antibody. Detection limit is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P56BT-1MG will be provided as 2 × 500 µg.
Target Information
IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen and is secreted at sites of acute and chronic inflammation, inducing a transcriptional inflammatory response via the interleukin-6 receptor alpha. Elevated IL-6 levels are associated with autoimmune diseases, infections (including COVID-19), diabetes mellitus susceptibility, and systemic juvenile rheumatoid arthritis. Diseases linked to IL-6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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