Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific의 IL-6 Polyclonal Antibody는 마우스 IL-6 검출용 고순도 항체로, Western Blot, ELISA, IHC 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 4 publications |
| Neutralization (Neu) | – | View 4 publications |
| Blocking Assay (BLOCK) | – | View 1 publication |
| In vitro Assay (IV) | – | View 3 publications |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-6 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930629 |
Product Specific Information
AA Sequence of recombinant protein:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-6 matrix.
Sandwich ELISA:
To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-6 (500-P56Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-6.
Western Blot:
To detect Murine IL-6 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen and is expressed at sites of acute and chronic inflammation. The cytokine induces transcriptional inflammatory responses through the interleukin 6 receptor, alpha. IL-6 is implicated in various inflammation-associated diseases, including diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated IL-6 levels have been observed in viral infections such as COVID-19. Diseases associated with IL-6 include Kaposi Sarcoma and Rheumatoid Arthritis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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