Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

Neutralization (Neu)

-

View 4 publications 4 publications

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Bacteria, Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine IL-6 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-6,uniProtId:P08505-1,ncbiNodeId:10090,antigenRange:25-211,antigenLength:211,antigenImageFileName:500-P56-100UG_IL-6_P08505-1_Rabbit.svg,antigenImageFileNamePDP:500-P56-100UG_IL-6_P08505-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930629

Product Specific Information

AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-6 matrix.

Sandwich ELISA: To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-6 (500-P56Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-6.

Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.