Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-6 matrix.

Sandwich ELISA:
To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-6 (500-P56Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-6.

Western Blot:
To detect Murine IL-6 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen and is expressed at sites of acute and chronic inflammation. The cytokine induces transcriptional inflammatory responses through the interleukin 6 receptor, alpha. IL-6 is implicated in various inflammation-associated diseases, including diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated IL-6 levels have been observed in viral infections such as COVID-19. Diseases associated with IL-6 include Kaposi Sarcoma and Rheumatoid Arthritis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.