Thermo Fisher Scientific IL-5 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG.

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-5. Anti-Murine IL-5-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect Murine IL-5 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-5 (500-P55) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-5.

Western Blot
To detect Murine IL-5 by Western Blot analysis, use at a concentration of 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Murine IL-5 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information
500-P55BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. It is a main regulator of eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 production is associated with asthma and hypereosinophilic syndromes.
The IL-5 receptor is a heterodimer whose beta subunit is shared with receptors for IL-3 and CSF2 (GM-CSF). IL-5, IL-4, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q31, regulated by long-range elements over 120 kb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.