Thermo Fisher Scientific IL-7 Polyclonal Antibody, PeproTech
Mouse IL-7에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA, IHC 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. PeproTech Biotinylated Anti-Murine IL-7과 함께 샌드위치 ELISA에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | – |
| Blocking Assay (BLOCK) | – | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-7 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930635 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-7. Anti-Murine IL-7-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-7 matrix.
Sandwich ELISA:
To detect Murine IL-7 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-7 (500-P57Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-7.
Western Blot:
To detect Murine IL-7 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine IL-7 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7 receptor (IL-7R) to promote the differentiation of hematopoietic stem cells into lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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