Thermo Fisher Scientific NOTCH1 Recombinant Rabbit Monoclonal Antibody (SJ205)
NOTCH1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포 발현 시스템을 통해 생산됩니다. 단백질 A로 정제된 액상 항체로, 4°C 또는 -20°C에서 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| Flow Cytometry (Flow) | 1:50–1:100 | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| SDS-PAGE | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SJ205 |
| Immunogen | Synthetic peptide within Human Notch 1 aa 2,481–2,530 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809374 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Consistent performance across lots
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The Notch gene family encodes transmembrane proteins with multiple EGF-like repeats in the extracellular region. Four Notch genes (Notch1–4) have been identified in mammals and are implicated in nervous system differentiation and other developmental processes.
Delta and Serrate proteins act as ligands for Notch1. Mature Notch proteins form heterodimeric receptors derived from cleavage of precursor Notch proteins into an extracellular subunit (NEC) and a transmembrane subunit (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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