Thermo Fisher Scientific APAF1 Recombinant Rabbit Monoclonal Antibody (SY22-02)
Western blot, IHC, ICC/IF에 사용 가능한 재조합 토끼 단클론 항체. Human 및 Mouse에 반응하며, HEK293 세포 발현 시스템으로 생산. 높은 특이성과 재현성을 제공하며, 단백질 Apaf-1 연구용으로 적합. 단백질 A로 정제된 액상 포맷.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY22-02 |
| Immunogen | Synthetic peptide within Human Apaf-1 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809376 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Key advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. It contains multiple WD-40 domains, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC).
Upon binding cytochrome c and dATP, APAF1 forms an oligomeric apoptosome that activates caspase 9, leading to the caspase cascade responsible for apoptosis.
Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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