Thermo Fisher Scientific SUMO1 Recombinant Rabbit Monoclonal Antibody (SJ20-03)
SUMO1 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, IHC, ICC, Flow cytometry, ChIP 등 다양한 응용에 적합합니다. 동물 유래 성분이 없는 포뮬레이션으로 로트 간 일관성이 우수합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| ChIP assay (ChIP) | 0.5–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SJ20-03 |
| Immunogen | Synthetic peptide within Human SUMO-1 (aa 51–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809373 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems, developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
SUMO1 is a ubiquitin-like protein that can be covalently attached to target proteins as a monomer or lysine-linked polymer. This modification requires activation by the E1 complex (SAE1-SAE2), transfer to the E2 enzyme (UBE2I), and can be promoted by E3 ligases such as PIAS1–4, RANBP2, or CBX4. SUMO1-mediated post-translational modification regulates crucial cellular processes including nuclear transport, DNA replication and repair, mitosis, and signal transduction. It is also involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains can be polyubiquitinated, signaling proteasomal degradation. Additionally, SUMO1 may regulate genes involved in palate development, and mutations can lead to non-syndromic orofacial cleft 10.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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