Thermo Fisher Scientific RPSA Recombinant Rabbit Monoclonal Antibody (JF0955)
RPSA 단백질을 인식하는 recombinant rabbit monoclonal antibody로 높은 특이성과 재현성을 제공합니다. WB, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. HEK293 시스템에서 발현되며 Protein A로 정제된 고품질 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0955 |
| Immunogen | Synthetic peptide within Human 67kDa Laminin Receptor (aa 250–295) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809776 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Laminins are extracellular matrix glycoproteins forming the major noncollagenous component of basement membranes. They are involved in cell adhesion, differentiation, migration, signaling, neurite outgrowth, and metastasis.
The laminin receptor 1 (RPSA) is a high-affinity, non-integrin laminin-binding protein known as 67 kDa laminin receptor, 37 kDa precursor (37LRP), or p40 ribosome-associated protein.
This receptor’s amino acid sequence is highly conserved, signifying a key biological role. Upregulation of RPSA is associated with colon and lung carcinoma, correlating with invasive and metastatic phenotypes.
Multiple pseudogene copies exist due to retropositional events, and two alternatively spliced transcripts encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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