Thermo Fisher Scientific Glucocorticoid Receptor Recombinant Rabbit Monoclonal Antibody (JF0952)
Thermo Fisher의 Glucocorticoid Receptor Recombinant Rabbit Monoclonal Antibody (JF0952)는 인간, 마우스, 랫, 제브라피시에 반응하는 고특이성 항체입니다. WB 및 Flow Cytometry에 적합하며, HEK293 발현 시스템에서 생산된 재조합 단클론 항체로 높은 재현성과 감도를 제공합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:5,000
Flow Cytometry (Flow)
- Tested Dilution: 1:50–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0952 |
| Immunogen | Recombinant protein within Human Glucocorticoid Receptor aa 2–220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809780 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Glucocorticoid Receptor (NR3C1) acts as both a transcription factor and a regulator of other transcription factors. It exists in cytoplasmic complexes with heat shock proteins and immunophilins, translocating to the nucleus upon ligand binding.
It is expressed in multiple heart tissues including atria, ventricles, aorta, and septum.
Alternate splicing and promoter usage yield several transcript variants.
Mutations in NR3C1 cause glucocorticoid resistance. GR binds steroids such as dexamethasone with nanomolar affinity and requires chaperone complexes for activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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