Thermo Fisher Scientific MAdCAM1 Monoclonal Antibody (MECA-367), Biotin, eBioscience

Thermo Fisher Scientific MAdCAM1 Monoclonal Antibody (MECA-367), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Product Specific Information

The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50–60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and serves as a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in the homing of memory T cells to the gut lamina propria and recirculation of naive lymphocytes in Peyer’s patches and mesenteric lymph nodes.

Applications Reported:
MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation, and immunoblotting (WB).

Applications Tested:
The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes, and can be used at ≤1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). It is recommended to titrate the antibody for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with leukocyte β7 integrin LPAM-1 (α4β7), L-selectin, and VLA-4 (α4β1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, though the full-length nature of some variants remains undetermined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.