Thermo Fisher Scientific IgE Monoclonal Antibody (23G3), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• FITC
• Request custom conjugation

Product Specific Information

The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA, IgM).

Applications Reported: Flow cytometric analysis and ELISA.
Applications Tested:

• ELISA: 1:5,000–1:10,000 dilution
• Flow Cytometry: ≤ 0.25 µg/test (approximately 10⁵–10⁸ cells/test)

A test is defined as the amount of antibody required to stain a cell sample in a final 100 µL volume. Optimal antibody concentration should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IgE is one of the five classes of mammalian immunoglobulins, consisting of two epsilon heavy chains and two light chains. It plays a key role in type I hypersensitivity reactions (e.g., hay fever, asthma, hives, anaphylaxis) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE-Fc receptor complexes triggers degranulation, leading to allergic responses. IgE also contributes to immune defense against parasitic infections.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.