Thermo Fisher Scientific IgE Monoclonal Antibody (23G3), Biotin, eBioscience
마우스 IgE epsilon 사슬에 특이적인 단클론 항체로, Flow Cytometry 및 ELISA에 적합합니다. Biotin으로 결합되어 있으며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 알레르기 반응 연구 및 면역학적 분석에 유용합니다.
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카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | ≤ 0.25 µg/test | View 4 publications |
| ELISA | 1:5,000–1:10,000 | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23G3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466856 |
Additional Formats
Product Specific Information
The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA, IgM).
Applications Reported: Flow cytometric analysis and ELISA.
Applications Tested:
- ELISA: 1:5,000–1:10,000 dilution
- Flow Cytometry: ≤ 0.25 µg/test (approximately 10⁵–10⁸ cells/test)
A test is defined as the amount of antibody required to stain a cell sample in a final 100 µL volume. Optimal antibody concentration should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IgE is one of the five classes of mammalian immunoglobulins, consisting of two epsilon heavy chains and two light chains. It plays a key role in type I hypersensitivity reactions (e.g., hay fever, asthma, hives, anaphylaxis) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE-Fc receptor complexes triggers degranulation, leading to allergic responses. IgE also contributes to immune defense against parasitic infections.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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