Thermo Fisher Scientific CCL4 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CCL4 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1beta (CCL4). Anti-Murine MIP-1beta (CCL4)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine MIP-1beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1beta (500-P213) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine MIP-1beta.

Western Blot:
To detect Murine MIP-1beta by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1beta is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P213BT-1MG is provided as 2 × 500 µg.

Target Information

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family.
Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. It is produced by macrophages upon stimulation by bacterial endotoxins and recruits inflammatory cells at sites of inflammation.
CCL4 is produced by lymphocytes, macrophages, and dendritic cells. Both CCL4 and CCL3 participate in host defense against bacterial, viral, parasitic, and fungal pathogens by regulating trafficking and activation of inflammatory cells.
CCL4 selectively attracts CD4+ lymphocytes, while CCL3 attracts CD8+ lymphocytes. Both act as chemoattractants for B cells, eosinophils, and dendritic cells.
Processed CCL4 can down-modulate CCR5 receptor expression, inhibiting CCR5-mediated HIV-1 entry in T cells. CCL4 binds with high affinity to CCR5 receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.