Thermo Fisher Scientific RELM beta Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Murine RELMβ 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질을 면역원으로 사용했습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific RELM beta Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.25–1.0 µg/mL | 3 publications |
| In vitro Assay (IV) | – | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.0 kDa Recombinant Murine RELMβ |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929859 |
Product Specific Information
AA Sequence of recombinant protein:
MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMβ. Anti-Murine RELMβ-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Murine RELMβ by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine RELMβ (500-P215) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine RELMβ.
Western Blot:
To detect Murine RELMβ by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine RELMβ is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P215BT-1MG is provided as 2 × 500 µg.
Target Information
Adipose tissue functions as an energy reserve and secretes various physiologically important proteins, including LPL, leptin, Acrp30, perilipin, and resistin.
Resistin (also known as Adipocyte Secreted Factor, ADSF, or FIZZ3) is a cytokine secreted by adipocytes. It plays a role in insulin resistance and glucose intolerance in mice, and its expression is regulated by C/EBP alpha, CBP, and p300 coactivators. PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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