Thermo Fisher Scientific RELM alpha Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.25-1.0 µg/mL

View 3 publications 3 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 10.0 kDa Recombinant Murine RELMalpha if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RELM alpha,uniProtId:Q9EP95-1,ncbiNodeId:10090,antigenRange:24-111,antigenLength:111,antigenImageFileName:500-P214BT-25UG_RELM_alpha_Q9EP95-1_Rabbit.svg,antigenImageFileNamePDP:500-P214BT-25UG_RELM_alpha_Q9EP95-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929853

Product Specific Information

AA Sequence of recombinant protein: MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMalpha. Anti-Murine RELMalpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine RELMalpha (500-P214) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMalpha.

Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P214BT-1MG will be provided as 2 x 500 µg

Target Information

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.