Thermo Fisher Scientific RECA-1 Monoclonal Antibody (HIS52), eBioscience
쥐 내피세포 표면 항원(RECA-1)을 인식하는 단클론 항체로, IHC(F) 및 유세포분석에 적합합니다. 높은 특이성과 순도를 가지며, 비쥐 종과 교차 반응하지 않습니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 1–10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS52 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467212 |
Product Specific Information
The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of the HIS52 antigen is not known. HIS52 does not cross-react with other species tested besides rat, making it a specific phenotypic marker for rat endothelium.
Applications Reported:
The HIS52 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. No reactivity was observed in immunoblotting of PAGE-run lysates from endothelial cell cultures or stromal cell preparations.
Applications Tested:
Tested by immunohistochemistry of rat frozen sections and can be used at 1–10 µg/mL. It is recommended to titrate the antibody for optimal performance in each assay.
Additional Information:
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
RECA-1 is a cell surface antigen expressed by all rat endothelial cells. The endothelium forms the inner lining of blood vessels, acting as an interface between circulating blood and the vessel wall. Endothelial cells line the entire circulatory system, from the heart to the smallest capillaries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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