Thermo Fisher Scientific CD33 Monoclonal Antibody (HIM3-4), eBioscience

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HIM3-4

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PE-Cyanine5
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2864864

Product Specific Information

Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.

Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIM3-4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.