Thermo Fisher Scientific APEX2 Polyclonal Antibody
APEX2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. Human 시료에 반응하며, Protein A 및 항원 친화 크로마토그래피로 정제됨. PBS buffer에 보관되며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:25 | - |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 143–171 amino acids from the central region of human APEX2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2718461 |
Target Information
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents, or by DNA glycosylases that remove specific abnormal bases.
AP sites are pre-mutagenic lesions that can prevent normal DNA replication, so the cell contains systems to identify and repair such sites.
Class II AP endonucleases cleave the phosphodiester backbone 5′ to the AP site.
This gene encodes a protein shown to have weak class II AP endonuclease activity.
Most of the encoded protein is located in the nucleus, but some is also present in mitochondria.
This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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