Thermo Fisher Scientific SUMO-1/Sentrin-1 Polyclonal Antibody
Human Sentrin-1(SUMO-1) 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot에 최적화되어 있습니다. 세포질 및 핵 내 단백질 검출에 사용되며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the N-terminus of human Sentrin-1 (SUMO1, UniProt ID: P63165-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: HeLa cell lysates
- Cellular location: Cytoplasmic and Nuclear
Target Information
SUMO1 (Small Ubiquitin-like Modifier 1) is a ubiquitin-like protein that can be covalently attached to target proteins as a monomer or polymer. This modification requires activation by the E1 complex (SAE1-SAE2), conjugation via the E2 enzyme (UBE2I), and may be promoted by E3 ligases such as PIAS1-4, RANBP2, or CBX4.
SUMOylation plays crucial roles in nuclear transport, DNA replication and repair, mitosis, and signal transduction. SUMO1 is involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains can undergo polyubiquitination, signaling proteasomal degradation. Additionally, SUMO1 may regulate genes involved in palate development, and mutations can cause non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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