Thermo Fisher Scientific IL-4 Monoclonal Antibody (BVD6-24G2), FITC, eBioscience
마우스 IL-4를 인식하는 단클론 항체로, FITC 형광 표지되어 세포 내 유세포분석에 적합합니다. Rat IgG1, kappa 아이소타입이며, 0.5 µg/test로 최적화되어 있습니다. 액상 형태로 4°C 보관하며, 연구용으로 사용됩니다.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (BVD6-24G2), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | 0.5 µg/test | View 19 publications |
| ELISA | - | View 1 publication |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BVD6-24G2 |
| Immunogen | E. coli expressed mouse IL-4 (gluteraldehyde XL) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465387 |
Available Formats
Product Specific Information
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.
Reported applications include intracellular flow cytometric analysis.
Tested by intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures.
Recommended usage: ≤ 0.5 µg per test (100 µL final volume). Optimal titration is advised for best results.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Post-manufacturing filtration: 0.2 µm.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor.
IL-4 and IL-13 share overlapping functions through receptor binding.
STAT6 mediates IL-4 signaling and immune regulation.
The IL-4 gene is part of a cytokine cluster on chromosome 5q, near IL-13 and IL-5, regulated by long-range elements over ~120 kb.
A splice variant, IL-4 delta 2, is known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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