Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-20F3), FITC, eBioscience
마우스 IL-6를 인식하는 중화용 단클론 항체로, FITC 형광표지되어 Flow Cytometry 분석에 적합합니다. 0.5 µg/test 권장, 488 nm 여기/520 nm 방출 스펙트럼. 4℃ 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 13 publications |
| ELISA | - | View 6 publications |
| Neutralization | - | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MP5-20F3 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465393 |
Available Formats
- Unconjugated
- Brilliant Violet 650
- eFluor 450
- Functional Grade
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6) and functions as a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21–28 kDa cytokine produced by various cell types including monocytes, fibroblasts, and endothelial cells. Upon stimulation, macrophages, T cells, B cells, mast cells, glial cells, eosinophils, keratinocytes, and granulocytes also secrete IL-6. This cytokine influences antigen-specific immune responses, inflammation, and neuronal development, and is a major mediator of the acute phase reaction.
Applications Reported:
This antibody has been reported for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining with flow cytometric analysis. Recommended ≤0.5 µg per test (100 µL final volume). Cell numbers typically range from 10⁵–10⁸ per test. Antibody titration is recommended for optimal performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen inducing fever in autoimmune or infectious conditions. IL-6 is primarily expressed at sites of inflammation and mediates transcriptional inflammatory responses via the IL-6 receptor alpha. Dysregulation of IL-6 is associated with several inflammatory diseases such as diabetes mellitus, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and rheumatoid arthritis. Elevated IL-6 levels have been observed in viral infections including COVID-19 (SARS-CoV-2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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