Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-5H4), FITC, eBioscience
Mouse IL-2를 인식하는 JES6-5H4 clone의 FITC 결합 단일클론 항체로, Flow Cytometry 및 ELISA에 사용 가능. 0.5 µg/test로 최적화되어 있으며, 488 nm/520 nm 형광 특성. Affinity chromatography로 정제된 고품질 연구용 항체.
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Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-5H4), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | 0.5 µg/test | View 29 publications |
| ELISA | - | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES6-5H4 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465381 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine.
- Reported Applications: Intracellular staining and flow cytometric analysis
- Tested Applications: Intracellular staining and flow cytometric analysis (≤ 0.5 µg/test)
- Definition of test: Amount (µg) of antibody staining a cell sample in 100 µL final volume
- Recommended titration for optimal performance
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine crucial for proliferation of activated T cells, differentiation of B cells, NK cells, monocytes, and macrophages.
IL-2 signals through IL-2R, a heterotrimeric receptor complex sharing gamma chain with IL-4 and IL-7.
Monoallelic expression in mature thymocytes regulates precise gene expression.
Disruption of IL-2-like gene in mice causes ulcerative colitis-like disease, indicating its essential role in immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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