Thermo Fisher Scientific CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience
CD11b 활성화 특이 에피토프를 인식하는 인간 Mac-1 단클론 항체로, 휴지기 세포에는 결합하지 않고 활성화된 호중구 및 단핵구에 선택적으로 반응함. 유세포분석, 면역침전, 차단 및 기능적 분석에 사용 가능. 0.5 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 20 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | 2 publications |
| Functional Assay (Functional) | Assay-Dependent | 2 publications |
| Blocking Assay (BLOCK) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CBRM1/5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467111 |
Available Conjugated Formats
- Alexa Fluor 700
- APC
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. It binds to a subset of Mac-1 molecules on neutrophils and monocytes after stimulation but does not recognize resting myeloid cells. Mac-1 participates in adhesive cell interactions through its ligands.
The epitope recognized by CBRM1/5 localizes to the I domain on the alpha chain of Mac-1, near the ligand binding site. This antibody blocks Mac-1-dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to ICAM-1.
Low-level activation may occur during processing of freshly drawn blood, leading to some binding in unstimulated samples; however, higher levels are observed in activated samples.
Applications Reported: Flow cytometry, immunoprecipitation, and blocking of CD11b function.
For functional assays, use the Functional Grade purified CBRM1/5 (Product #16-0113).
Applications Tested: Tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. Recommended ≤1 µg/test (100 µL final volume). Cell numbers may vary (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimer (Mac-1, CR3) functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia. CD11b expression increases during monocyte maturation and varies among tissue macrophages.
Associated diseases include systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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