Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eBioscience
Mouse CD8a를 인식하는 53-6.7 클론 Rat IgG2a 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 분석에 사용 가능. 고순도(>90%)로 Affinity chromatography 정제. 0.25 µg/test로 최적화된 성능 제공. 연구용으로 T 세포 활성화 및 CD8+ 세포 분석에 적합.
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- 14-0081-8x (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 57 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 8 |
| Immunocytochemistry (ICC/IF) | - | 16 |
| Flow Cytometry (Flow) | 0.25 µg/test | 447 |
| Immunoprecipitation (IP) | Assay-Dependent | 1 |
| Neutralization (Neu) | - | 4 |
| Functional Assay (Functional) | - | 13 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Fruit fly, Hamster, Human, Insect, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467087 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugate Formats
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Yellow series (multiple wavelengths)
- eFluor series (450, 506, 615, 660)
- FITC
- Functional Grade
- NovaFluor Blue/Red/Violet/Yellow series
- PE, PE-Cyanine variants, PerCP series
- Super Bright series (436–780)
- Custom conjugation available upon request
Product Specific Information
The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8 is found on thymocytes, mature αβ T cells, γδ T cells, intestinal intraepithelial lymphocytes, and dendritic cells. It binds to MHC class I and associates with tyrosine kinase p56^lck, playing a role in T cell development and activation.
Applications Reported:
Used for flow cytometry, immunoprecipitation, and frozen tissue immunohistology. Also applied in T cell activation, CD8+ cell isolation/depletion, and ligand blocking.
For functional assays, use Functional Grade purified 53-6.7 (Product #16-0081).
Applications Tested:
Validated by flow cytometric analysis of mouse thymocytes and splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal performance requires empirical titration.
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein in the immunoglobulin receptor family. It acts as a co-receptor with TCR for MHC class I molecules, facilitating T cell signal transduction, differentiation, and activation. CD8 is expressed as CD8αβ heterodimers on T cells and as CD8αα homodimers on subsets of T cells, NK cells, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, inducing cytokine production and cytotoxic T lymphocyte (CTL) activation. CTLs are essential for pathogen and tumor clearance and are enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures.
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