Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series (multiple wavelengths)
• eFluor series (450, 506, 615, 660)
• FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow series
• PE, PE-Cyanine variants, PerCP series
• Super Bright series (436–780)
• Custom conjugation available upon request

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8 is found on thymocytes, mature αβ T cells, γδ T cells, intestinal intraepithelial lymphocytes, and dendritic cells. It binds to MHC class I and associates with tyrosine kinase p56^lck, playing a role in T cell development and activation.

Applications Reported:
Used for flow cytometry, immunoprecipitation, and frozen tissue immunohistology. Also applied in T cell activation, CD8+ cell isolation/depletion, and ligand blocking.
For functional assays, use Functional Grade purified 53-6.7 (Product #16-0081).

Applications Tested:
Validated by flow cytometric analysis of mouse thymocytes and splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal performance requires empirical titration.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein in the immunoglobulin receptor family. It acts as a co-receptor with TCR for MHC class I molecules, facilitating T cell signal transduction, differentiation, and activation. CD8 is expressed as CD8αβ heterodimers on T cells and as CD8αα homodimers on subsets of T cells, NK cells, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, inducing cytokine production and cytotoxic T lymphocyte (CTL) activation. CTLs are essential for pathogen and tumor clearance and are enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.