Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), Biotin, Functional Grade, eBioscience

Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), Biotin, Functional Grade

Applications and Tested Dilution

Product Specifications

Product Specific Information

The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10), an ~18 kDa cytokine known as Cytokine Synthesis Inhibitory Factor (CSIF).
In mice, IL-10 is produced mainly by Th2 cells, B1 cells, macrophages, and keratinocytes. It inhibits Th1 cytokine synthesis, suppresses T-cell proliferation, and acts as a co-stimulatory signal for mast cells and developing thymocytes.

Applications Reported:
Functional Grade Biotin anti-mouse IL-10 antibody (clone JES5-16E3) is used as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA), which increases cytokine measurement sensitivity by extending their in vivo half-lives.

For detailed methodology, see:
Finkelman, F., Morris, S., Orekhova, T., and Sehy, D. (2003). The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology, Unit 6.28. John Wiley and Sons, New York.

Applications Tested:
The Functional Grade Biotin JES5-16E3 antibody is LAL-tested and endotoxin-free. It has been used as the detection antibody in sandwich ELISA for mouse IL-10, in combination with JES5-2A5 (14-7102) for capture and recombinant mouse IL-10 (14-8101) as standard.
Recommended ELISA detection concentration: 0.5–2 µg/mL.
Standard curve range: 4000 pg/mL – 30 pg/mL.

Endotoxin: Less than 0.05 ng/µg antibody (LAL assay).
Storage and Handling: Use in a sterile environment.

Target Information

IL-10 is a cytokine primarily produced by monocytes and lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages.
IL-10 enhances B cell survival, proliferation, and antibody production, and inhibits NF-κB activity. It plays a key role in JAK-STAT signaling regulation.
Knockout studies in mice show IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.