Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Ultra Violet 737

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, and activated B cells. Activated T cells also express this antigen. CD80 binds to T cell surface antigens CD28 and CD152 (CTLA-4), crucial in T-B cell communication and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤0.125 µg/test (100 µL final volume). Cell numbers range from 10⁵–10⁸ cells/test. Optimal titration recommended.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, suitable for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:
Samples can be stored up to 3 days at 4°C in IC Fixation Buffer or Fix/Lyse Solution. Compatible with short-term methanol-based fixation (<1 hour).

Excitation/Emission: 355 nm / 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and essential for T cell activation. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. It provides regulatory signals for T lymphocytes via CD28 and CTLA4 binding.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures.

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