Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Custom conjugation available

Product Specific Information

The 2E7 monoclonal antibody reacts with mouse CD103 (Integrin alpha E).
CD103 non-covalently associates with integrin beta7 and is expressed on intraepithelial lymphocytes, a subset of peripheral lymphocytes, and dendritic epidermal T cells.
E-cadherin binds to CD103 and mediates lymphocyte homing to the intestinal epithelium.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse lymph node cells at ≤1.0 µg/test (100 µL final volume).
Cell number may vary from 10⁵–10⁸ cells/test; titration recommended for optimal performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for cytometers equipped with an ultraviolet (355 nm) laser.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation; protect vial and samples from light.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
BUV737 is compatible with short-term methanol-based fixation (<1 hour).

Excitation / Emission / Laser:
355 nm / 732 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated cells.
It consists of a 150 kDa alpha chain and a 120 kDa beta chain, forming a heterodimeric integrin complex.
CD103 expression is found in 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs.
It plays a role in adhesion and activation of intestinal intraepithelial lymphocytes (IEL).
Associated diseases include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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