Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 737, eBioscience
인간 CD123(IL-3 수용체 α사슬)을 인식하는 6H6 단클론 항체로, Brilliant Ultra Violet 737 형광체로 표지된 제품입니다. 유세포 분석용으로 최적화되어 있으며, 355 nm UV 레이저에서 732 nm 파장을 방출합니다. 실험 시 광노출을 피하고 4°C에서 보관합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 737, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 737, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | Brilliant Ultra Violet™ 737 |
| Excitation / Emission Max | 350 / 740 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2895992 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 805
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60–70 kDa transmembrane protein binds IL-3 with low affinity by itself, and with CD131 forms a high-affinity receptor complex. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).
Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for use with ultraviolet (355 nm) lasers. Ensure your cytometer supports this fluorochrome.
Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.
Light Sensitivity:
Protect from light to prevent photo-induced oxidation.
Fixation Compatibility:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333). Brilliant Ultra Violet™ 737 is compatible with short-term methanol fixation (<1 hour).
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family, forming a heterodimer with the common beta (CDw131) subunit. It mediates IL-3 signal transduction and becomes activated upon IL-3-induced heterodimerization. IL-3Ra is overexpressed in acute myeloid leukemia (AML) blasts compared to normal hematopoietic cells, representing a potential therapeutic target.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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