Thermo Fisher Scientific VISTA Monoclonal Antibody (MIH64), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• APC
• Brilliant UV 615
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• NovaFluor Yellow 730
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

This MIH64 monoclonal antibody recognizes mouse VISTA (also known as B7-H5 or PD-H1).
It is validated for flow cytometry on both native and paraformaldehyde-fixed cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell count may vary from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for use with cytometers equipped with a 355 nm UV laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (SB-4401) or
• Brilliant Stain Buffer (00-4409-75)
  to minimize nonspecific polymer interactions.

Handling Notes:

• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using:
  • IC Fixation Buffer (00-8222) or
  • 1-step Fix/Lyse Solution (00-5333)
• Methanol compatibility: Compatible with short-term methanol fixation (<1 hour); avoid long-term storage in methanol-containing buffers.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is encoded by the VSIR gene.
VISTA is expressed primarily on hematopoietic cells, with high levels on myeloid cells and lower levels on T cells, but typically absent on B cells.
It suppresses proliferation and cytokine production of CD4⁺ and CD8⁺ T cells, making it a potential target for cancer immunotherapy.
Reported ligands include CD28H, VSIG-3, and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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