Thermo Fisher Scientific Mouse anti-Human IgG (CH2 domain) Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

0.5 µg/mL

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

R10Z8E9

Immunogen

Polyclonal Human IgG

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538406

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

This antibody does not react with baboon, cynomolgus monkey, marmoset, mouse, rat or rhesus monkey.

Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognizes Human IgG. Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.

Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognizes Human IgG.Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.