Thermo Fisher Scientific CD34 Monoclonal Antibody (QBEND/10)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 59 publications 59 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

1:100-1:200

-

Immunoprecipitation (IP)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

QBEND/10

Immunogen

Human endothelial cell membrane vesicles if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD34,uniProtId:P28906-1,ncbiNodeId:9606,antigenRange:1-385,antigenLength:385,antigenImageFileName:MA5-16926_CD34_P28906-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16926_CD34_P28906-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538403

Product Specific Information

This antibody does not react with bovine, dog, rat or sheep.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. A suggested positive control for immunohistochemical applications is tonsil or bone marrow. This product does not require antigen retreival using heat treatment or protein digestion. However staining of paraffin-embedded, formalin-fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer.

Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.