Thermo Fisher Scientific Human SCF Recombinant Protein

Applications

Western Blot (WB)

• Tested Dilution: Assay-dependent

Immunohistochemistry (IHC)

• Tested Dilution: Assay-dependent

ELISA

• Tested Dilution: Assay-dependent

Functional Assay

• Tested Dilution: Assay-dependent
• Activity confirmed in 5 publications

Control (Ctrl)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free

Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to 0.1–1.0 mg/mL to regain full activity. Make further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.

Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles.

Target Information

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF to the c-Kit receptor leads to receptor homodimerization and activation of signaling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase.
SCF expression promotes mast cell survival and the release of histamine, pro-inflammatory cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting its role as a therapeutic target in inflammatory diseases such as asthma.
At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.