Thermo Fisher Scientific Human SCF Recombinant Protein
인간 SCF 재조합 단백질로, E. coli 발현 시스템에서 생산된 고순도(>95%) 단백질입니다. 세포 증식 및 기능 분석용으로 사용되며, ED50 < 10 ng/mL의 높은 생물학적 활성을 보입니다. 동결건조 형태로 제공되며, 재구성 후 -20°C 이하에서 보관합니다.
- 카탈로그번호
- PHC211x (4개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human SCF Recombinant Protein
Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Immunohistochemistry (IHC)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Functional Assay
- Tested Dilution: Assay-dependent
- Activity confirmed in 5 publications
Control (Ctrl)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA |
| Molecular Weight | 18.54 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 < 10 ng/mL; determined by the dose-dependent proliferation of human MO-7e cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to 0.1–1.0 mg/mL to regain full activity. Make further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF to the c-Kit receptor leads to receptor homodimerization and activation of signaling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase.
SCF expression promotes mast cell survival and the release of histamine, pro-inflammatory cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting its role as a therapeutic target in inflammatory diseases such as asthma.
At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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