Thermo Fisher Scientific Human EPO (Erythropoietin) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

View 5 publications 5 publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

CHO cells

Amino acid sequence

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Molecular weight

30.4 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

Specific Activity = 150,000 IU/mg; determined by measuring normocythaemic mice.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

0.006mg citric acid/0.59mg sodium citrate with 0.58mg NaCl

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, deionized water to a concentration of 12,000-120,000 IU/mL. Further dilutions can be made in aqueous buffers. For long term storage, EPO solutions should be supplemented with carrier protein, such as 0.1% HSA or BSA.

Storage: This lyophilized preparation is stable at room temperature for three weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.

Target Information

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.