Thermo Fisher Scientific Human IFN-alpha A (alpha 2a) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Molecular weight

19.2 kDa

Class

Recombinant

Type

Protein

Purity

≥95%

Activity

Specific Activity = 2.5x10e8 units/mg; Assay used to measure bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981 Convenient Assay for Interferons, J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon, in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce 50% cytopathic effect. The units are determined with respect to the international reference standard for human interferon alpha (Hu-IFN-α provided by the National Institutes of Health [see Pestka, S. (1986) Interferon Standards and General Abbreviations, in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23]. The activity was determined using bovine MDBK cells challenged with vesicular stomatitis virus (VSV).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Dilution Recommendation: Further dilutions should be made in medium or buffered solution containing carrier protein such as PBS with 0.1% BSA.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.