Thermo Fisher Scientific Human TNF-alpha Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free
Storage: Store the liquid human TNF-alpha at ≤ -20°C. Upon thawing, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell activity, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers of two, three, or five noncovalently linked units but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice suggest TNF alpha has neuroprotective functions and induces tumor necrosis when injected into tumor-bearing mice. It plays roles in immune responses to bacterial, viral, parasitic, and fungal infections and contributes to tumor cell cytolysis or cytostasis, synergistic with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.