Thermo Fisher Scientific Human TNF-alpha Recombinant Protein
인간 TNF-alpha 재조합 단백질로, E. coli에서 발현된 고순도(>95%) 시료입니다. 다양한 어세이(WB, IHC, ELISA, Functional Assay)에 사용 가능하며, 강력한 생물학적 활성을 보입니다. 연구용으로만 사용되며, -20°C 이하에서 보관하십시오.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Molecular Weight | 17.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.02 - 0.05 ng/mL; determined by dose-dependent cytotoxicity on L929 cells in presence of actinomycin D |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | 40 mM Tris, pH 8 |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Dry ice |
Product Specific Information
Carrier-Free
Storage: Store the liquid human TNF-alpha at ≤ -20°C. Upon thawing, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell activity, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers of two, three, or five noncovalently linked units but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice suggest TNF alpha has neuroprotective functions and induces tumor necrosis when injected into tumor-bearing mice. It plays roles in immune responses to bacterial, viral, parasitic, and fungal infections and contributes to tumor cell cytolysis or cytostasis, synergistic with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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