Thermo Fisher Scientific B7-H4 Monoclonal Antibody (188), eBioscience
Mouse B7-H4 단백질을 인식하는 Rat IgG2b 단클론 항체로, Flow Cytometry 분석에 최적화됨. 0.5 µg/test 농도로 사용 가능하며, T세포 활성 억제 연구에 활용. 고순도(>90%) 및 0.2 µm 필터링 처리된 액상 형태.
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Thermo Fisher Scientific B7-H4 Monoclonal Antibody (Clone 188), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View publication |
| Neutralization (Neu) | - | View publication |
| Functional Assay | - | View publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467769 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 (also known as B7S1, B7X). Cross-reactivity to other proteins has not been determined.
B7-H4 is a member of the B7 family that inhibits T-cell activation, cell cycle progression, and IL-2 production.
Double staining experiments using clones 9 and 188 indicate distinct epitopes. Clone 188 stains mouse B7-H4 transfected cells but not spleen cells.
Applications reported: Flow cytometric analysis of transfected cells.
Applications tested: ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.
Target Information
B7-H4 (B7x, VTCN1) is a costimulatory protein acting as a negative regulator of T-cell mediated immunity.
It delivers inhibitory signals to T-cells, preventing proliferation and IL-2 production.
B7-H4-deficient mice show minimal immune impact, indicating its role in fine-tuning immune responses.
Expressed on activated T-cells, B-cells, monocytes, and dendritic cells.
Aberrant expression is linked to lung, breast, and ovarian cancers.
Overexpression may suppress T-cell immunity and promote tumor progression.
VTCN1 is a potential biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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