Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1 or NTPDase), an integral membrane protein with two transmembrane domains forming a homotetramer. CD39 is the major ectoenzyme of the immune system, converting extracellular ATP to ADP or AMP, and often works with CD73.
Expression occurs on activated lymphocytes, subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes. CD39 and CD73 are also found on regulatory T cells (Treg), facilitating their migration into inflamed areas with high ATP levels. Expression on Foxp3⁺CD4⁺ cells ranges from 25–45%.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on human peripheral blood cells. Recommended use: 5 µL (0.8 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Adjust titration for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates use Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue)

Target Information

CD39 is a 70–100 kDa cell surface antigen expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It has intrinsic ecto-ATPase activity and acts as a late activation antigen on T and B cells.

For Research Use Only. Not for diagnostic use or resale without authorization.

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