Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Blue 690

Applications

• Flow Cytometry (Flow)
• Immunohistochemistry (IHC)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1, NTPDase), an integral membrane protein with two transmembrane domains forming a homotetramer. CD39 removes extracellular ATP by converting it to ADP or AMP, functioning with CD73 to hydrolyze ATP. It is expressed on activated lymphocytes, subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes.

CD39 and CD73 are found on regulatory T cells (Treg), aiding their migration into inflamed tissues. Expression on Foxp3⁺CD4⁺ cells ranges from 25–45%.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometry and immunohistochemical staining
Applications Tested: Pre-titrated and validated for flow cytometry using human peripheral blood cells at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before mixing with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor Technology:
Based on Phiton™ nucleic acid dye structures, engineered for reduced spillover and improved spectral performance.
Excitation: 494 nm; Emission: 585 nm; Laser: 488 nm (Blue).

Target Information

CD39 is a 70–100 kDa cell surface antigen expressed on B cells, monocytes, T cell clones, and weakly on granulocytes. It has ecto-ATPase activity and acts as a late activation antigen, upregulated on activated T and B cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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