Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤5 µL (1.0 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa surface glycoprotein expressed on most thymocytes, T-helper cells, and at low levels on monocytes. CD4 acts as a receptor for HIV. The OKT4 antibody recognizes a different epitope than RPA-T4 and does not cross-block with it.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at ≤5 µL (1.0 µg)/test. Optimal titration is recommended.

Note:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.

NovaFluor Conjugates:
Based on Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.

• Excitation: 494 nm
• Emission: 585 nm
• Laser: 488 nm (Blue)

Target Information

CD4 antigen participates in MHC class II recognition and acts as a co-receptor for HIV. It is mainly expressed in T-helper cells and to a lesser extent in monocytes, macrophages, and dendritic cells. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues. It contributes to early T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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