Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 615 / 737 / 805
• Brilliant Violet 421 / 605 / 786
• eFluor 450
• FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5), also known as Burkitt lymphoma receptor 1 (BLR1). CD185 is a seven-transmembrane G protein-coupled receptor identified in Burkitt’s lymphoma. It is expressed on B cells, CD4⁺ T cells (excluding Th1 and Th2), and a subset of memory T cells (CD45RO⁺).

In tonsil tissue, CD185 is expressed on nearly all CD4⁺ cells with activation markers such as CD69 and ICOS, and plays a role in antibody induction through interaction with B cells. CD185 is crucial in lymphocyte trafficking, particularly for T cell migration into B cell follicles in response to CXCL13, serving as a marker for follicular helper T cells.

• Cross-reactivity: Rhesus macaque
• Applications Reported: Flow cytometry
• Applications Tested: Pre-diluted; recommended 5 µL (0.5 µg)/test
• Instrument Compatibility: For cytometers with a violet (405 nm) laser
• Excitation / Emission: 440 / 479 nm
• Fixation Compatibility: Not compatible with methanol-based fixation

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (SB-4401-42) or
• Brilliant Stain Buffer™ (00-4409-75)

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen™.

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), belongs to the CXC chemokine receptor family. It is a G protein-coupled receptor for chemokine BLC and is expressed in lymphatic tissues such as lymph node follicles and spleen.

CXCR5 plays an essential role in B cell migration and is expressed in human blood, salivary gland, and various animal tissues (lymphatic organs, bone marrow, brain, liver, spleen, and tonsil). As a GPCR, it participates in signal transduction through G-proteins and serves as a pharmacological target in multiple research areas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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