Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), eFluor 570, eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
41-4777-82 | - | Thermo Fisher Scientific 41-4777-82 FOXP3 Monoclonal Antibody (236A/E7), eFluor 570, eBioscience 100 ug pk | 재고문의 | pk | 644,000원 | - | 708,400원 | |
41-4777-80 | - | Thermo Fisher Scientific 41-4777-80 FOXP3 Monoclonal Antibody (236A/E7), eFluor 570, eBioscience 25 ug pk | 재고문의 | pk | 286,000원 | - | 314,600원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Western Blot (WB)
-
View 1 publication 1 publication
Immunohistochemistry (IHC)
1:25
View 22 publications 22 publications
Immunohistochemistry (Paraffin) (IHC (P))
Assay-Dependent
View 3 publications 3 publications
Immunohistochemistry (Frozen) (IHC (F))
5 µg/mL
View 1 publication 1 publication
Immunocytochemistry (ICC/IF)
5 µg/mL
View 1 publication 1 publication
Immunoprecipitation (IP)
-
View 1 publication 1 publication
Radioimmune Assays (RIA)
-
View 1 publication 1 publication
Product Specifications
Species Reactivity
Human, Non-human primate, Rhesus monkey
Published species
Human
Host/Isotype
Mouse / IgG1, kappa
Recommended Isotype Control
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 570, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
236A/E7
Conjugate
eFluor™ 570 eFluor™ 570 eFluor™ 570
View additional formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- Request custom conjugation
Excitation/Emission Max
556/569 nm View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573608
Product Specific Information
Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in scurfy
(sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.
Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.
The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776-40). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.
Applications Reported: This 236A/E7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This 236A/E7 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high or low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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