Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, eFluor 570, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a secondary reagent in flow cytometry and microscopy. It does not recognize other rat isotypes nor cross-react with mouse IgG2a or any mouse isotype antibodies.

Applications Reported:
This antibody is suitable for immunocytochemistry and immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections.

Applications Tested:
Tested by immunocytochemistry on fixed and permeabilized cells stained first with a rat IgG2a anti-mouse antibody. Recommended concentration ≤10 µg/mL. Antibody should be titrated for optimal performance.

Filter Recommendation:
For eFluor® 570 conjugate, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Standard Alexa Fluor® 555 or TRITC filters are acceptable.
Excitation: 555 nm; Emission: 570 nm

Target Information

Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of rat antibodies. Secondary antibodies enhance assay versatility and sensitivity via signal amplification, as multiple secondary antibodies can bind a single primary antibody. These reagents are typically generated by immunizing host animals with pooled immunoglobulins from the target species and purified through immunoaffinity chromatography or conjugation techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats

• eFluor 615
• eFluor 660
• FITC
• PE
• PerCP-eFluor 710
• Custom conjugation available upon request

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